Application
Western blot analysis of Frataxin expression in 293 whole cell lysate.Application
Western blot analysis of Frataxin expression in non-transfected (A) and human Frataxin transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Product Name | Frataxin Antibody (V-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Friedreich ataxia is a progressive neurodegenerative disorder caused by loss of function mutations in the frataxin gene. The human frataxin gene maps to chromosome 9q13 (1).The frataxin gene encodes a mitochondrial protein of the same name (2). Frataxin assembles into a stable homopolymer with iron-binding capabilities (3). When expressed in E. Coli, human frataxin binds iron atoms at a rate of 10 iron atoms per 1 molecule of the frataxin polymer (3). Thus, frataxin appears to function in some capacity for iron-storage for the mitochondria (3). Frataxin may also function as an activator of oxidative phosphorylation to increase mitochondrial membrane potential and elevate cellular ATP (4). Frataxin is expressed in tissues with high metabolic activity including heart, liver and brown fat (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Frataxin of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Frataxin of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 2395 |
|---|
Application
Western blot analysis of Frataxin expression in 293 whole cell lysate.
Application
Western blot analysis of Frataxin expression in non-transfected (A) and human Frataxin transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
