Application
Western blot analysis of SIMP expression in HL-60 (A) and CCRF-CEM (B) whole cell lysates.| Product Name | SIMP Antibody (K-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SIMP (source of immunodominant MHC-associated peptides), also known as STT3B (STT3, subunit of the oligosaccharyltransferase complex, homolog B), is an 826 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and belongs to the STT3 family. Expressed in liver, heart, placenta, kidney, brain, muscle and pancreatic tissue, SIMP exists as a component of the multi-protein oligosaccharyltransferase (OST) complex and functions to catalyze the N-glycosylation of target proteins. More specifically, SIMP mediates the transfer of high mannose oligosaccharides from lipid-linked oligosaccharide donors to target asparagine residues on polypeptide chains. The gene encoding SIMP maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SIMP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of SIMP of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of SIMP of human origin |
| Entrez Gene | 201595 |
|---|
Application
Western blot analysis of SIMP expression in HL-60 (A) and CCRF-CEM (B) whole cell lysates.| Application Notes | recommended for detection of SIMP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
