Application
Western blot analysis of RDH5 expression in non-transfected: (A) and human RDH5 transfected: (B) 293T whole cell lysates.| Product Name | RDH5 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Retinol dehydrogenase 5 (RDH5), also known as 11-cis retinol dehydrogenase (11-cis RDH) or RDH1, is a 318 amino acid protein belonging to the short-chain dehydrogenases/reductases (SDR) family. Highly expressed in the retinal pigment epithelium and localized to the membrane, RDH5 catalyzes the final step in the biosynthesis of 11-cis retinal (11-cis retinaldehyde), the universal chromophore of visual pigment, from all-trans retinol (vitamin A). RDH5 has been shown to be active in the presence of NAD as a cofactor, but not in the presence of NADP. Mutations in the gene encoding RDH5 lead to fundus albipunctatus (FA), a rare form of stationary night blindness characterized by delay in the regeneration of cone and rod photopigments. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RDH5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 111-150 mapping within an internal region of RDH5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 111-150 mapping within an internal region of RDH5 of human origin |
| Entrez Gene | 5959 |
|---|
Application
Western blot analysis of RDH5 expression in non-transfected: (A) and human RDH5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of RDH5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
