Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.| Product Name | ANKRD53 Antibody (Q-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases, such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKRD53 (ankyrin repeat domain 53) is a 530 amino acid protein that contains three ANK repeats. Conserved in chimpanzee, dog, cow, mouse and zebrafish, ANKRD53 is encoded by a gene that maps to human chromosome 2p13.3. As the second largest human chromosome, chromosome 2 makes up approximately 8% of the human genome and contains 237 million bases encoding over 1,400 genes. Chromosome 2 contains a probable vestigial second centromere, as well as vestigial telomeres, which gives credence to the hypothesis that human chromosome 2 formed as a result of an ancient fusion of two ancestral chromosomes, which are still present in modern day apes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of ANKRD53 isoforms 1 and 2 of human origin by WB, IF and ELISA non cross-reactive with other ANKRD family members | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of ANKRD53 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of ANKRD53 of human origin |
| Entrez Gene | 79998 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.| Application Notes | recommended for detection of ANKRD53 isoforms 1 and 2 of human origin by WB, IF and ELISA non cross-reactive with other ANKRD family members |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
