Application
Western blot analysis of Nek8 expression in A2058 whole cell lysate.| Product Name | Nek8 Antibody (K-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. Nek8 [NIMA (never in mitosis gene a)- related kinase 8], also known as serine/threonine-protein kinase Nek8, NPHP9, NEK12A, MGC138445, Jck or NEK12A, is a mitotic regulator that plays a significant role in maintaining renal tubular integrity. Nek8 localizes to cytoplasm and is abundant in thyroid, adrenal gland and skin, with overexpression in breast tumors and infiltrating ductal carcinomas. Moderate levels of Nek8 has been found in mucinous adenocarcinoma. Nek8 contains one protein kinase domain and five RCC1 repeats. Nek8 mutations correlate to juvenile autosomal recessive polycystic kidney disease in humans. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Nek8 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Nek8 of human origin by WB, IP and ELISA |
| Entrez Gene | 284086 |
|---|
Application
Western blot analysis of Nek8 expression in A2058 whole cell lysate.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
