Application
Western blot analysis of hnRNP E1 expression in non-transfected 293T: (A), mouse hnRNP E1 transfected 293T: (B), CCRF-CEM (C) and K-562 (D) whole cell lysates.Application
Western blot analysis of hnRNP E1 expression in Hep G2 whole cell lysate.| Product Name | hnRNP E1 Antibody (Z-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of hnRNP E1 of human origin by WB, IP and ELISA |
| Modification Notes | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP E1 and E2. hnRNP E1 may function in the cytoplasm as a translational regulatory protein, while hnRNP E2 stabilizes mRNA to enhance polioviral mRNA translation. hnRNP M is involved in pre-mRNA splicing and in stress-induced transient splicing arrest. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of hnRNP E1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of hnRNP E1 of human origin by WB, IP and ELISA |
| Entrez Gene | 5093 |
|---|
Application
Western blot analysis of hnRNP E1 expression in non-transfected 293T: (A), mouse hnRNP E1 transfected 293T: (B), CCRF-CEM (C) and K-562 (D) whole cell lysates.
Application
Western blot analysis of hnRNP E1 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
