Application
Western blot analysis of Ribosomal Protein L13 expression in non-transfected 293T: (A), human Ribosomal Protein L13 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Ribosomal Protein L13 expression in non-transfected 293T: (A), human Ribosomal Protein L13 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Ribosomal Protein L13 expression in Jurkat whole cell lysate.| Product Name | Ribosomal Protein L13 Antibody (U-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ribosomal Protein L13 of mouse, rat, human and zebrafish origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L13, also known as RPL13 or BBC1 (breast basic conserved protein 1), is a 211 amino acid protein that is a component of the 60S subunit. Localized to the cytoplasm and expressed ubiquitously, Ribosomal Protein L13 belongs to the L13E family of ribosomal proteins and functions in protein synthesis. In addition, the gene encoding Ribosomal Protein L13 is expressed at high levels in benign breast lesions. Like most ribosomal proteins, Ribosomal Protein L13 exists as multiple processed pseudogenes that are scattered throughout the genome. Due to alternative splicing events and/or alternative polyadenylation, various isoforms exist for Ribosomal Protein L13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ribosomal Protein L13 of mouse rat human and zebrafish origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Ribosomal Protein L13 of mouse, rat, human and zebrafish origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 6137 |
|---|
Application
Western blot analysis of Ribosomal Protein L13 expression in non-transfected 293T: (A), human Ribosomal Protein L13 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Ribosomal Protein L13 expression in non-transfected 293T: (A), human Ribosomal Protein L13 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Ribosomal Protein L13 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
