Application
Western blot analysis of Dbp5 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing nuclear localization.| Product Name | DDX19B Antibody (J-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of DDX19B of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. DEAD box proteins contain two conserved RecA-like domains that adopt different open structures in the absence of nucleic acid and closed structure when bound to RNA and ATP. They also exhibit RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. DEAD box RNA helicase DEAD5 (Dbp5), also known as DEAD box protein 19B (DDX19B), is a 479 amino acid protein belonging to the DEAD box family. Localized to the cytoplasm and nuclear envelope, Dbp5 participates in the export of mRNA from the nucleus to the cytoplasm. Dbp5 is activated by interactions mediated by Gle1 and is inhibited by Nup214. Two named isoforms of Dbp5 exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of DDX19B of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of DDX19B of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 11269 |
|---|
Application
Western blot analysis of Dbp5 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing nuclear localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
