Application
Western blot analysis of GCX-1 expression in Jurkat whole cell lysate.| Product Name | GCX-1 Antibody (L-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GCX-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Human reproduction is controlled by the hypothalamic-pituitary gonadal axis (HPGA) that is laid down early in fetal development. GCX-1 (Granulosa cell HMG box protein 1), also designated TOX2 (TOX high mobility group box family member 2), is a 488 amino acid transcription activator that is restricted to expression in the hypothalamus, pituitary, ovary, testis and uterus. This expression pattern in the HPGA suggests that this nuclear protein is likely related to specific events in reproduction, particularly in the female. GCX-1 contains a HMG-box domain, which is commonly found in proteins that function as intercellular regulators and transcriptional co-regulators, and are found to be involved in important events such as sex determination and in the regulation of T cell differentiation. There are two isoforms of GCX-1 which are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GCX-1 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of GCX-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 84969 |
|---|
Application
Western blot analysis of GCX-1 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
