Application
Western blot analysis of AKAP 7 expression in Hep G2 whole cell lysate.| Product Name | AKAP 7 Antibody (F-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of AKAP 7 of human origin by WB, IP and ELISA |
| Modification Notes | The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the regulatory (R) subunits (RI and RII) of PKA interact with specific R-anchoring proteins designated AKAPs (A-kinase anchoring proteins). AKAP 7 ( A-kinase anchor protein 7), also known as AKAP18, is a 104 amino acid protein that belongs to the AKAP family. AKAP 7 is expressed in brain, heart, lung, pancreas and skeletal muscle. AKAP 7 binds PKA to the plasma membrane, and permits functional coupling to the L-type calcium channel. Four isoforms exist due to alternative splicing events. It has been suggested that the © isoform binds RI and may be responsible for positioning PKA via RI and/or RII to regulate PKA-mediated gene transcription in both somatic cells and oocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of AKAP 7 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of AKAP 7 of human origin by WB, IP and ELISA |
| Entrez Gene | 9465 |
|---|
Application
Western blot analysis of AKAP 7 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
