Application
Western blot analysis of Membralin expression in non-transfected 293T: (A), human Membralin transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Membralin expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Product Name | Membralin Antibody (B-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Membralin of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The gene encoding human Membralin, C19orf6, localizes to chromosome 19p13.3. It contains 11 exons, which encode at least two splice variants in human cancer. Membralin is a multi-pass membrane protein and exists either as a long or short form. The long form of Membralin comprises all 11 exons, while the short form contains all exons except exon 10. Expression of different Membralin isoforms depends on tissue type. The long form is expressed in ovarian and colorectal carcinomas, whereas the short form is expressed in breast or pancreatic carcinomas. Because Membralin expression in ovarian carcinomas is highest in serous carcinomas as compared to surface epithelium carcinomas, expression of Membralin may be useful as a novel tumor-associated marker in ovarian serous carcinomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Membralin of mouse rat human and zebrafish origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Membralin of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 91304 |
|---|
Application
Western blot analysis of Membralin expression in non-transfected 293T: (A), human Membralin transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Membralin expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
