Application
Western blot analysis of GRB7 expression in A-431 (A) and EGF treated A-431 (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse ovary tissue showing membrane and cytoplasmic localization.| Product Name | GRB7 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of GRB7 of human origin |
| Modification Notes | Many growth factors function by binding receptors with intrinsic tyrosine kinase activity (1,2). Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and the phosphotyrosine-containing receptor sequences (3,4). GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N-terminus (5,6). GRB7 maps to the region on mouse chromosome 11 containing the Neu gene (6). This region of mouse chromosome 11 is syntenic to an area of human chromosome 17q that is frequently amplified in breast cancer (6,7). Moreover, GRB7 is amplified and over-expressed in breast cancer and is found in a complex with Neu gp185 (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GRB7 and, to a lesser extent, with GRB10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of GRB7 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of GRB7 of human origin |
| Entrez Gene | 2886 |
|---|
Application
Western blot analysis of GRB7 expression in A-431 (A) and EGF treated A-431 (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse ovary tissue showing membrane and cytoplasmic localization.| Application Notes | recommended for detection of GRB7 and, to a lesser extent, with GRB10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
