Application
Western blot analysis of Synaptotagmin I phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Product Name | p-Synaptotagmin II Antibody (Thr 202) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Thr 202 phosphorylated Synaptotagmin II of mouse and rat origin and correspondingly Thr 199 phosphorylated Synaptotagmin II of mouse, rat, human and zebrafish origin by WB and IP; also reactive with additional species, includin |
| Modification | Phosphoralated |
| Modification Notes | Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis and are involved in neurotransmitter secretion from small secretory vesicles. Calcium binds to Synaptotagmin I which triggers neurotransmitter release at the synapse. Synaptotagmin II is phosphorylated by WNK1 in a process that regulates calcium-dependent interactions. Synaptotagmin III is involved in calcium-dependent exocytosis of secretory vesicles in endocrine cells and neurons. Synaptotagmin IV is expressed in neuronal tissues, and has the highest mRNA levels in the hippocampus. The proximity of the Synaptotagmin IV gene to markers of several psychiatric disorders suggest an involvement of synaptotagmin IV in human disease. Synaptotagmin V is a dense-core vesicle-specific protein that regulates a specific type of calcium-regulated secretion. Synaptotagmin VI interacts with adaptor protein-2 in a calcium-independent manner. Synaptotagmin VII is widely expressed in non-neuronal tissues. Mouse, rat and human Synaptotagmin II are phosphorylated by WNK1 on Thr 202, which is located within the calcium binding domain and, upon phosphorylation, allows Synaptotagmin II to bind to phospholipid vesicles. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Thr 202 phosphorylated Synaptotagmin II of mouse and rat origin and correspondingly Thr 199 phosphorylated Synaptotagmin II of mouse rat human and zebrafish origin by WB and IP also reactive with additional species includin |
|---|---|
| Tissue Specificity | recommended for detection of Thr 202 phosphorylated Synaptotagmin II of mouse and rat origin and correspondingly Thr 199 phosphorylated Synaptotagmin II of mouse, rat, human and zebrafish origin by WB and IP; also reactive with additional species, including bovine and canine |
| Entrez Gene | 127833 |
|---|
Application
Western blot analysis of Synaptotagmin I phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
