Application
Western blot analysis of CKR-5 expression in non-transfected 293T: (A), human CKR-5 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CKR-5 expression in Jurkat whole cell lysate.| Product Name | CKR-5 Antibody (H-185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 66-250 of C-C chemokine receptor gene 5 (CKR-5) of human origin |
| Modification Notes | C-C or ∫ chemokine family members are characterized by a pair of adjacent cysteine residues and serve as potent chemoattractants and activators of monocytes and T cells. C-C chemokine receptor family members include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7, CKR-8, CKR-9, CKR-10 and the Duffy blood group antigen. Each of these receptors are G protein coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation. However, this receptor family has also been shown to facilitate viral infection. Termed a “coreceptor”, CKR-5, along with CD4, has been shown to be a major receptor for HIV. CKR-5 tends to associate with macrophage-tropic viruses, such as macrophage tropic HIV-1, while CKR-2B and CKR-3 bind a minority of viruses. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CKR-5 of human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 66-250 of C-C chemokine receptor gene 5 (CKR-5) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 66-250 of C-C chemokine receptor gene 5 (CKR-5) of human origin |
| Entrez Gene | 1234 |
|---|
Application
Western blot analysis of CKR-5 expression in non-transfected 293T: (A), human CKR-5 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CKR-5 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of CKR-5 of human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
