Application
Western blot analysis of FBL12 expression in A-431 (A), H69AR (B) and 804G (C) whole cell lysates.| Product Name | FBL12 Antibody (H-273) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 54-326 mapping at the C-terminus of FBL12 of human origin |
| Modification Notes | F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune responses, signaling cascades and developmental events) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, I˚B-å and ∫-catenin, for proteasomal degradation. FBL12 (F-box and leucine-rich repeat protein 12), also designated FBXL12, is a 326 amino acid protein that contains one F-box domain, five LRR (leucine-rich) repeats and exists as two alternatively spliced isoforms. FBL12 interacts with both Skp1 and CUL-1, and is encoded by a gene that maps to human chromosome 19p13.2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FBL12 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 54-326 mapping at the C-terminus of FBL12 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 54-326 mapping at the C-terminus of FBL12 of human origin |
Application
Western blot analysis of FBL12 expression in A-431 (A), H69AR (B) and 804G (C) whole cell lysates.| Application Notes | recommended for detection of FBL12 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
