Application
Western blot analysis of NOBOX expression in MIA PaCa-2 (A) and ES-2 (B) whole cell lysates.Application
Western blot analysis of NOBOX expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear staining.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining.| Product Name | NOBOX Antibody (D-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against NOBOX of human origin |
| Modification Notes | Early ovarian folliculogenesis is characterized by the breakdown of germ cell clusters and formation of primordial follicles. The cessation of ovarian function under the age of 40 years results in premature ovarian failure (POF) and is accompanied by amenorrhea, hypoestrogenism and elevated serum gonadotropin concentrations. 1% of all women are affected by POF, and mutations in a few genes, including inhibinå, fsh receptor and the LH/choriogonadotropin receptor have been linked to POF. In addition, several germ cell specific genes and downstream transcription factors are thought to play and important role in human oogenesis. NOBOX (newborn ovary homeobox gene), an ooctye-specific homeobox gene, is a critical protein involved in early folliculogenesis. Missense mutations have been shown to cause nonsyndromic ovarian failure by disrupting the NOBOX proteins ability to bind to NOBOX DNA-binding element (NBE), and thereby inhibiting its regulation of Pou5f1 and GDF-9. NOBOX expression in the ovary is oocyte specific, but it shows expression in adult testis and pancreas as well. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NOBOX of human origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~1:5000 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against NOBOX of human origin |
|---|---|
| Tissue Specificity | raised against NOBOX of human origin |
| Entrez Gene | 135935 |
|---|
Application
Western blot analysis of NOBOX expression in MIA PaCa-2 (A) and ES-2 (B) whole cell lysates.
Application
Western blot analysis of NOBOX expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear staining.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of NOBOX of human origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~1:5000 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
