Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of human (A) and mouse (B) recombinant TNF-R1.Application
Western blot analysis of TNF-R1 expression in non-transfected: (A) and mouse TNF-R1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | TNF-R1 Antibody (H-271) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 30-301 including the extracellular domain of TNF-R1 of human origin |
| Modification Notes | Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors (1). These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types (1,2). The majority of TNF functions are primarily mediated through TNF-R1, while signaling through TNF-R2 occurs less extensively and is confined to cells of the immune system (1). Both of these proteins belong to the growing TNF and nerve growth factor (NGF) receptor superfamily, which includes FAS, CD30, CD27 and CD40 (3). The members of this superfamily are type I membrane proteins that share sequence homology confined to the extracellular region (2). TNF-R1 shares a motif coined the “death domain” with FAS and three structurally unrelated signaling proteins, TRADD, FADD and RIP (1,3–8). This “death domain” is required for transduction of the apoptotic signal (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TNF-R1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 30-301 including the extracellular domain of TNF-R1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 30-301 including the extracellular domain of TNF-R1 of human origin |
| Entrez Gene | 7132 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of human (A) and mouse (B) recombinant TNF-R1.
Application
Western blot analysis of TNF-R1 expression in non-transfected: (A) and mouse TNF-R1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of TNF-R1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
