Application
Western blot analysis of NNP-1B expression in HEK293 whole cell lysate (A) and HeLa (B), Jurkat (C), K-562 (D) and NIH/3T3 (E) nuclear extracts.| Product Name | NNP-1B Antibody (K-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of NNP-1B of human origin |
| Modification Notes | NNP-1B (novel nuclear protein 1B), also known as RRP1-like protein B (Rrp1b) in mouse and rat, is a 758 amino acid protein belonging to the RRP1 family. Localized to the nucleolus, NNP-1B is expressed as two isoforms produced by alternative splicing. Ubiquitously expressed, the N-terminus of NNP-1B is 80% homologous to fellow RRP1 family member NNP-1, but has a divergent C-terminus. NNP-1 has been found to play an important role in the generation of 28S rRNA in the late processing steps of ribosome biogenesis. At the end of mitosis, nucleolar proteins assemble in a sequential order during the rebuilding of the nucleolus. NNP-1 assembles after Fibrillarin and C23, and simultaneously with B23 and POP1 in the prenucleolar body pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NNP-1B of human origin and Rrp1b of mouse and rat origin by WB, IP, IF and ELISA non cross-reactive with other RRP family members also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of NNP-1B of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of NNP-1B of human origin |
| Entrez Gene | 23076 |
|---|
Application
Western blot analysis of NNP-1B expression in HEK293 whole cell lysate (A) and HeLa (B), Jurkat (C), K-562 (D) and NIH/3T3 (E) nuclear extracts.| Application Notes | recommended for detection of NNP-1B of human origin and Rrp1b of mouse and rat origin by WB, IP, IF and ELISA non cross-reactive with other RRP family members also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
