Application
Western blot analysis of CHAC1 expression in HeLa (A), NIH/3T3 (B), MIA PaCa-2 (C) and C2C12 (D) whole cell lysates and mouse pancreas tissue extract (E).| Product Name | CHAC1 Antibody (E-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of CHAC1 of human origin |
| Modification Notes | CHAC1 (ChaC, cation transport regulator homolog 1 (E. coli)) is a 264 amino acid cytoplasmic protein that belongs to the ChaC family. Induced by chemical activators of the unfolded protein response (UPR) pathway, including DTT, tunicamycin and thapsigargin, CHAC1 is a pro-apoptotic member of this pathway and may mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Overexpression of CHAC1 may lead to an increase in apoptosis, while suppression of CHAC1 results in subdued apoptosis. Existing as two alternatively spliced isoforms, the gene encoding CHAC1 maps to human chromosome 15q15.1. Encoding more than 700 genes, chromosome 15 is made up of approximately 106 million base pairs and comprises about 3% of the human genome. Angelman, Prader-Willi and Marfan syndromes are associated with loss of function or deletion of genes that are encoded by chromosome 15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CHAC1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with CHAC2 also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of CHAC1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of CHAC1 of human origin |
| Entrez Gene | 79094 |
|---|
Application
Western blot analysis of CHAC1 expression in HeLa (A), NIH/3T3 (B), MIA PaCa-2 (C) and C2C12 (D) whole cell lysates and mouse pancreas tissue extract (E).| Application Notes | recommended for detection of CHAC1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with CHAC2 also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
