Application
Western blot analysis of Eme1 expression in 293 showing nontransfected (A) and transfected (B) whole cell lysates.| Product Name | Eme1 Antibody (N-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Eme1 of human origin by WB, IP and ELISA |
| Modification Notes | Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with MUS81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The MUS81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that MUS81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Eme1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Eme1 of human origin by WB, IP and ELISA |
| Entrez Gene | 146956 |
|---|
Application
Western blot analysis of Eme1 expression in 293 showing nontransfected (A) and transfected (B) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
