Application
Western blot analysis of MIF4GD expression in non-transfected: (A) and mouse MIF4GD transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MIF4GD expression in non-transfected: (A) and mouse MIF4GD transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MIF4GD expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Product Name | MIF4GD Antibody (F-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against MIF4GD of human origin |
| Modification Notes | MIF4GD (MIF4G domain containing), also known as MIFD, AD023 or SLIP1, is a 222 amino acid protein that contains one MIF4G domain. Localized to both the nucleus and the cytoplasm, MIF4GD plays a role in the replication-dependent translation of histone mRNAs, which differ from most eukaryotic mRNAs in that they end with a stem-loop instead of a poly-A tail. Specifically, MIF4GD interacts with SLBP, eIF4G and DAP-5. Via its interaction with SLBP, MIF4GD is thought to be tethered to the stem loops of histone mRNAs where it may facilitate the circularizing of the mRNAs, thereby enhancing their translation. Depletion of MIF4GD results in reduced histone translation and may lead to cell death, suggesting that MIF4GD plays an important role in cell survival. Two isoforms of MIF4GD exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MIF4GD of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against MIF4GD of human origin |
|---|---|
| Tissue Specificity | raised against MIF4GD of human origin |
| Entrez Gene | 57409 |
|---|
Application
Western blot analysis of MIF4GD expression in non-transfected: (A) and mouse MIF4GD transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MIF4GD expression in non-transfected: (A) and mouse MIF4GD transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MIF4GD expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Application Notes | recommended for detection of MIF4GD of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
