Application
Western blot analysis of HELB expression in Jurkat whole cell lysate.| Product Name | HELB Antibody (P-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against HELB of human origin |
| Modification Notes | HELB (helicase B), also known as hDHB (human DNA helicase B), is a 1,087 amino acid ATPase and 5’-3’ DNA helicase. Due to a preference for ATP and dATP as substrates, HELB binds strongly to single-stranded DNA only in the absence of ATP. HELB has been shown to bind to RPA 70 kDa subunit and at least 2 subunits of the polymerase å-primase complex during DNA replication. Upon DNA damage, HELB is thought to be phosphorylated by either Atm or ATR. When a dominant-negative mutant of the HELB protein was injected into the nucleus of early G1 phase cells, DNA synthesis was halted, suggesting that HELB is necessary for cell cycle progression. HELB is expressed highly in thymus and testis and is present at lower levels in kidney, spleen, brain and liver. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of HELB of human origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against HELB of human origin |
|---|---|
| Tissue Specificity | raised against HELB of human origin |
| Entrez Gene | 92797 |
|---|
Application
Western blot analysis of HELB expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of HELB of human origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
