Application
Western blot analysis of Ezrin phosphorylation in non-transfected 293T: (A), human Ezrin transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of phosphorylated Ezrin expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.| Product Name | p-Ezrin Antibody (Thr 566) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 566 of Ezrin of human origin |
| Modification | Phosphoralated |
| Modification Notes | The microvillar core protein Ezrin is a tyrosine-phosphorylated protein found in epidermal growth factor-stimulated A-431 carcinoma cells (3). Ezrin is phosphorylated transiently to a high level on tyrosine residues 146 and 354 (1,2). One of the sites, Tyrosine 146, lies in the amino-terminal region of homology that is common to the Protein 4.1-Talin-Ezrin protein family (1,4). This tyrosine residue and its vicinal amino acids are conserved throughout the family which includes Radixin, Moesin, and the two phosphotyrosine phosphatases, PTP H1 and PTP MEG (1). Another phosphorylation site is Tyrosine 354, which is localized within the alpha-helical domain of Ezrin (1). Cytoplasmic signaling may result in activation of Ezrin in tyrosine phosphorylation and this suggests that Ezrin has qualities that might play a role in modulation of cell shape and adhesion (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Thr 566 phosphorylated Ezrin of human and mouse origin and correspondingly phosphorylated Thr 567 of rat origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 566 of Ezrin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 566 of Ezrin of human origin |
| Entrez Gene | 7430 |
|---|
Application
Western blot analysis of Ezrin phosphorylation in non-transfected 293T: (A), human Ezrin transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of phosphorylated Ezrin expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.| Application Notes | recommended for detection of Thr 566 phosphorylated Ezrin of human and mouse origin and correspondingly phosphorylated Thr 567 of rat origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
