Application
Western blot analysis of XLF expression in non-transfected: (A) and mouse XLF transfected: (B) 293T whole cell lysates.| Product Name | XLF Antibody (FL-299) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-299 representing full length XLF of human origin |
| Modification Notes | XLF (XRCC4-like factor), also known as non-homologous end-joining factor 1 (NHEJ1) or cernunnos, is a 295 amino acid protein belonging to the XLF family. There are two main repair pathways for DNA double-strand breaks: homologous recombination (HR) and non-homologous end-joining (NHEJ). In the latter pathway, the Ku-70/Ku-86 heterodimer binds the DNA ends together and the DNA-PK catalytic subunits are recruited. Then the DNA ends are processed by DNA processing enzymes, such as Artemis. The binding is finalized through DNA Ligase IV, which acts in a complex with XRCC4 and XLF to stabilize the repair. Thus, it is believed that XLF interacts with DNA Ligase IV and XRCC4 to constitute the enzymatic core of the NHEJ machinery. Two named isoforms of XLF exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of XLF of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-299 representing full length XLF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-299 representing full length XLF of human origin |
| Entrez Gene | 79840 |
|---|
Application
Western blot analysis of XLF expression in non-transfected: (A) and mouse XLF transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of XLF of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
