Application
Western blot analysis of phosphorylated β-catenin expression in untreated (A) and Calyculin A-treated (B) SW626 whole cell lysates.| Product Name | p-β-catenin Antibody (Thr 41/Ser 45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 41/Ser 45 of β-catenin of human origin |
| Modification | Phosphoralated |
| Modification Notes | The catenins, å, ∫ and ©, are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. ∫-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. ∫-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3∫, result in accumulation of the ∫-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of ∫-catenin signaling that directly binds a phosphorylated Ser-Pro motif next to the APC-binding site in ∫-catenin, inhibiting the interaction with APC and increasing ∫-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of ∫-catenin in tumors such as breast cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Thr 41/Ser 45 phosphorylated β-catenin of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 41/Ser 45 of &beta -catenin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 41/Ser 45 of β-catenin of human origin |
| Entrez Gene | 1499 |
|---|
Application
Western blot analysis of phosphorylated β-catenin expression in untreated (A) and Calyculin A-treated (B) SW626 whole cell lysates.| Application Notes | recommended for detection of Thr 41/Ser 45 phosphorylated β-catenin of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
