Application
Western blot analysis of MC-CPA expression in non-transfected (A) and human MC-CPA transfected (B) whole cell lysates.| Product Name | MC-CPA Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 316-365 mapping near the C-terminus of MC-CPA of human origin |
| Modification Notes | Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MC-CPA (Mast cell carboxypeptidase A precursor and mature protein) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 316-365 mapping near the C-terminus of MC-CPA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 316-365 mapping near the C-terminus of MC-CPA of human origin |
| Entrez Gene | 1359 |
|---|
Application
Western blot analysis of MC-CPA expression in non-transfected (A) and human MC-CPA transfected (B) whole cell lysates.| Application Notes | recommended for detection of MC-CPA (Mast cell carboxypeptidase A precursor and mature protein) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
