Application
Western blot analysis of AGTRAP expression in non-transfected: (A) and human AGTRAP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AGTRAP expression in non-transfected: (A) and human AGTRAP transfected: (B) 293T whole cell lysates.| Product Name | AGTRAP Antibody (FL-159) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-159 representing full length AGTRAP of human origin |
| Modification Notes | AGTRAP (angiotensin II receptor-associated protein), also known as ATRAP, is a transmembrane protein that localizes to the golgi apparatus, the endoplasmic reticulum (ER), endocytotic vesicles and perinuclear vesicular structures. Highly expressed in heart, kidney, pancreas and thyroid, AGTRAP functions as a negative regulator of the Angiotensin II type I receptor (AT1). AGTRAP controls receptor internalization and receptor desensitization events (such as phosphorylation) and, through this control, decreases Angiotensin II signaling, thereby reducing rates of cell proliferation and Angiotensin II-stimulated transcriptional activity. AGTRAP is 159 amino acids in length and is able to bind RACK1 (receptor for activated C kinase 1); an association that is thought to help recruit AGTRAP to AT1. Two isoforms of AGTRAP exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AGTRAP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-159 representing full length AGTRAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-159 representing full length AGTRAP of human origin |
Application
Western blot analysis of AGTRAP expression in non-transfected: (A) and human AGTRAP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AGTRAP expression in non-transfected: (A) and human AGTRAP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of AGTRAP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
