Application
Antibodies tested include p-Raf-1 (Ser 259): (A,B,C) and Raf-1 (540): (D,E,F).Application
Antibodies tested include p-Raf-1 (Ser 259): (A, B, C) and Raf-1 (540): (D, E, F).Application
Antibodies tested include p-Raf-1 (Ser 259): preincubated with cognate phosphorylated peptide (A) and p-Raf-1 (Ser 259): (B,C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-Raf-1 Antibody (Ser 259) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 259 of Raf-1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 259 phosphorylated Raf-1 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 259 of Raf-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 259 of Raf-1 of human origin |
| Entrez Gene | 5894 |
|---|
Application
Antibodies tested include p-Raf-1 (Ser 259): (A,B,C) and Raf-1 (540): (D,E,F).
Application
Antibodies tested include p-Raf-1 (Ser 259): (A, B, C) and Raf-1 (540): (D, E, F).
Application
Antibodies tested include p-Raf-1 (Ser 259): preincubated with cognate phosphorylated peptide (A) and p-Raf-1 (Ser 259): (B,C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 259 phosphorylated Raf-1 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
