Application
Western blot analysis of CYB561D2 expression in mouse liver tissue extract.Application
Western blot analysis of CYB561D2 expression in non-transfected: (A) and mouse CYB561D2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CYB561D2 expression in non-transfected: (A) and human CYB561D2 transfected: (B) 293T whole cell lysates.| Product Name | CYB561D2 Antibody (C-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a C-terminal cytoplasmic domain of CYB561D2 of human origin |
| Modification Notes | CYB561D2 (Cytochrome b561 domain-containing protein 2), also known as putative tumor suppressor protein 101F6, is a 222 amino acid highly hydrophobic protein that contains six membrane spanning domains. Localized to endosomes and endoplasmic reticulum of the perinuclear region, CYB561D2 contains 2 heme-binding sites, through which it reduces ferric ions. CYB561D2 is ubiquitously expressed, with highest levels in lung, liver and kidney. Though expression of CYB561D2 is abundant in normal lung bronchial epithileal cells, CYB561D2 is absent in most lung cancers. The gene encoding CYB561D2 maps to human chromosome 3p21.3, a region that is prone to genetic alterations and is associated with frequent and early allele loss in many human cancers. Exogenous expression of CYB561D2 leads to the induction of caspase-independent apoptotic and autophagic pathways, resulting in the synergistic killing of tumor cells. This suggests an important role for CYB561D2 as a therapeutic intervention in cancer treatment. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CYB561D2 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with family member CYB561D1 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a C-terminal cytoplasmic domain of CYB561D2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a C-terminal cytoplasmic domain of CYB561D2 of human origin |
| Entrez Gene | 11068 |
|---|
Application
Western blot analysis of CYB561D2 expression in mouse liver tissue extract.
Application
Western blot analysis of CYB561D2 expression in non-transfected: (A) and mouse CYB561D2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CYB561D2 expression in non-transfected: (A) and human CYB561D2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CYB561D2 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with family member CYB561D1 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
