Application
Western blot analysis of RUNDC3A expression in non-transfected: (A) and human RUNDC3A transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).| Product Name | RUNDC3A Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 276-340 mapping within an internal region of RUNDC3A of human origin |
| Modification Notes | RUNDC3A (RUN domain containing 3A), also known as RPIP-8 (Rap2-interacting protein 8) or RAP2IP, is a 446 amino acid protein that is thought to act as an effector protein of RAP2A in neuronal cells. A member of the RUNDC3 family, RUNDC3A contains one RUN domain and undergoes alternative splicing events to produce four isoforms. RUNDC3A is expressed in testis, brain, kidney and liver, and is encoded by a gene that maps to human chromosome 17q21.31. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RUNDC3A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 276-340 mapping within an internal region of RUNDC3A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 276-340 mapping within an internal region of RUNDC3A of human origin |
| Entrez Gene | 10900 |
|---|
Application
Western blot analysis of RUNDC3A expression in non-transfected: (A) and human RUNDC3A transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).| Application Notes | recommended for detection of RUNDC3A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
