| Product Name | I-SceI Antibody (FL-86) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 150-235 mapping at the C-terminus of I-SceI of Saccharomyces cerevisiae origin |
| Modification Notes | Group I intron-encoded endonucleases, like I-SceI, represent a new class of double strand cutting endonucleases which generate double strand breaks in site-specific sequences (1,2). Despite its small size, I-SceI initiates intron homing by recognizing and specifically cleaving a large intronless DNA sequence (3). I-SceI binds to its substrate in monomeric form (3). The I-SceI restriction site is absent from most prokaryotic and eukaryotic genomes (4). The mitochondrial I-SceI has an 18-bp recognition sequence and, therefore, has a very low probability of cutting DNA, even within large genomes (5). Double-strand breaks can be initiated by the I-SceI endonuclease at a predetermined location in the genome and the breaks can be repaired with a donor molecule homologous regions flanking the breaks (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of I-SceI of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 150-235 mapping at the C-terminus of I-SceI of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | raised against amino acids 150-235 mapping at the C-terminus of I-SceI of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of I-SceI of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
