Application
Western blot analysis of ATF-7 expression in non-transfected 293T: (A), mouse ATF-7 transfected 293T: (B) and RAW 264.7 (C) whole cell lysates.Application
Western blot analysis of ATF-7 expression in NIH/3T3 (A) and RAW 264.7 (B) nuclear extracts.| Product Name | ATF-7 Antibody (E-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of ATF-7 of human origin |
| Modification Notes | Eukaryotic gene transcription is regulated by sequence-specific transcription factors, which bind modular cis acting promoter and enhancer elements. The cAMP response element (CRE) consists of the palindromic octanucleotide TGACGTCA. There are several CRE binding proteins within the ATF/CREB family, including CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. A novel basic leucine zipper (bZIP) protein, designated ATF-7, is closely related to members of the ATF/CREB family of bZIP proteins. ATF-7 physically interacts with the PRL-1 protein-tyrosine phosphatase (PTPase), which is a predominately nuclear, farnesylated PTPase. ATF-7 homodimers bind specifically to CRE elements. ATF-7 is expressed in a number of different tissues and is expressed in association with differentiation. ATF-7 and PRL-1 interact with each other through the bZIP region of ATF-7 and the phosphatase domain of PRL-1. In addition, PRL-1 is able to dephosphorylate ATF-7 in vitro. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| recommended for detection of ATF-7 of mouse, rat and human origin by WB and IF also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of ATF-7 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of ATF-7 of human origin |
Application
Western blot analysis of ATF-7 expression in non-transfected 293T: (A), mouse ATF-7 transfected 293T: (B) and RAW 264.7 (C) whole cell lysates.
Application
Western blot analysis of ATF-7 expression in NIH/3T3 (A) and RAW 264.7 (B) nuclear extracts.| Application Notes | recommended for detection of ATF-7 of mouse, rat and human origin by WB and IF also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
