Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic staining of bone marrow poietic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Flt-3/Flk-2 immunoprecipitated from THP-1 whole cell lysate with Flt-3/Flk-2 (SF1.340): and detected with Flt-3/Flk-2 (C-20): .Application
Western blot analysis of Flt-3/Flk-2 expression in THP-1 whole cell lysate.| Product Name | Flt-3/Flk-2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Flt-3/Flk-2 of human origin |
| Modification Notes | Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Flt-3/Flk-2 p160 and p130 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Flt-3/Flk-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Flt-3/Flk-2 of human origin |
| Entrez Gene | 2322 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic staining of bone marrow poietic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Flt-3/Flk-2 immunoprecipitated from THP-1 whole cell lysate with Flt-3/Flk-2 (SF1.340): and detected with Flt-3/Flk-2 (C-20): .
Application
Western blot analysis of Flt-3/Flk-2 expression in THP-1 whole cell lysate.| Application Notes | recommended for detection of Flt-3/Flk-2 p160 and p130 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
