Application
Western blot analysis of APPL1 expression in non-transfected: (A) and mouse APPL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of APPL1 expression in non-transfected 293T: (A), mouse APPL1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of APPL1 expression in 3T3-L1 (A), A-673 (B), A-10 (C) and HeLa (D) whole cell lysates.| Product Name | APPL1 Antibody (H-96) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 614-709 mapping at the C-terminus of APPL1 of human origin |
| Modification Notes | The APPL family of proteins are involved in linking, trafficking and signaling downstream of tyrosine kinase receptors. APPL1, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; APPL; or DCC interacting protein 13å (DIP13å), and APPL2, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 2 or DCC interacting protein 13∫ (DIP13∫), are involved in the coupling of epidermal growth factor (EGF) signaling and chromatin remodeling in the nucleus. They associate with GTPase Rab 5 and are released from the plasma membrane and translocated to the nucleus. In the nucleus, APPL1 and APPL2 associate with NuRD/MeCP1 and are essential for cell growth and proliferation. APPL1 is also involved in Akt regulation, binding the kinase domains of Akt1 and Akt2; neurotrophin receptor signaling via association with GIPC and Trk A; and it associates with follicle-stimulating hormone receptor (FSHR) and the cata-lytic subunit of type 1A PI 3-kinase. APPL1 is highly expressed in heart, ovary, skeletal muscle and pancreas. APPL1 shares 54% homology with APPL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of APPL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 614-709 mapping at the C-terminus of APPL1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 614-709 mapping at the C-terminus of APPL1 of human origin |
| Entrez Gene | 26060 |
|---|
Application
Western blot analysis of APPL1 expression in non-transfected: (A) and mouse APPL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of APPL1 expression in non-transfected 293T: (A), mouse APPL1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of APPL1 expression in 3T3-L1 (A), A-673 (B), A-10 (C) and HeLa (D) whole cell lysates.| Application Notes | recommended for detection of APPL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
