Application
Western blot analysis of PGRMC1 expression in Hep G2 (A), SK-BR-3 (B) and MCF7 (C) whole cell lysates and rat liver (D) and human liver (E) tissue extracts.Application
Western blot analysis of PGRMC1 expression in non-transfected: (A) and human PGRMC1 transfected: (B) 293T whole cell lysates.| Product Name | PGRMC1 Antibody (H-46) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-46 mapping at the N-terminus of PGRMC1 of human origin |
| Modification Notes | PGRMC1 (progesterone receptor membrane component 1), also known as MPR, is a 195 amino acid single-pass membrane protein that localizes to both the endoplasmic reticulum and to the microsome and contains one cytochrome b5 heme-binding domain. Expressed in a variety of tissues with highest expression in kidney and liver, PGRMC1 functions as a receptor for progesterone, a steroid hormone that is involved in embryonic development and is crucial for proper female maturation. The gene encoding PGRMC1 maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination, as an X and a Y chromosome lead to normal male development, while two copies of an X chromosome lead to normal female development. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PGRMC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-46 mapping at the N-terminus of PGRMC1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-46 mapping at the N-terminus of PGRMC1 of human origin |
| Entrez Gene | 10857 |
|---|
Application
Western blot analysis of PGRMC1 expression in Hep G2 (A), SK-BR-3 (B) and MCF7 (C) whole cell lysates and rat liver (D) and human liver (E) tissue extracts.
Application
Western blot analysis of PGRMC1 expression in non-transfected: (A) and human PGRMC1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PGRMC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
