Application
Western blot analysis of Fatso expression in SW-13 (A) and SK-N-MC (B) whole cell lysates.Application
Western blot analysis of Fatso expression in non-transfected: (A) and human Fatso transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Fatso expression in SW-13 whole cell lysate.Application
Western blot analysis of Fatso expression in mouse pancreas tissue extract (A) and MIA PaCa-2 whole cell lysate (B).| Product Name | Fatso Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 252-505 mapping at the C-terminus of Fatso of human origin |
| Modification Notes | Fatso, also known as FTO or KIAA1752, is a 505 amino acid protein that has an N-terminal nuclear localization signal. Expressed in a variety of tissues, with highest levels present in brain and pancreatic tissue, Fatso exists as four alternatively spliced isoforms, one of which is associated with a predisposition to childhood and adult obesity. Due to its involvement in the development of obesity, Fatso is associated with an increased BMI and may be involved in the pathogenesis of type 2 diabetes. The gene encoding Fatso maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Fatso of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 252-505 mapping at the C-terminus of Fatso of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 252-505 mapping at the C-terminus of Fatso of human origin |
| Entrez Gene | 79068 |
|---|
Application
Western blot analysis of Fatso expression in SW-13 (A) and SK-N-MC (B) whole cell lysates.
Application
Western blot analysis of Fatso expression in non-transfected: (A) and human Fatso transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Fatso expression in SW-13 whole cell lysate.
Application
Western blot analysis of Fatso expression in mouse pancreas tissue extract (A) and MIA PaCa-2 whole cell lysate (B).| Application Notes | recommended for detection of Fatso of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
