Application
Western blot analysis of CtBP1 expression in non-transfected: (A) and human CtBP1 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of CtBP1 expression in non-transfected: (A) and mouse CtBP1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.Application
Western blot analysis of CtBP expression in Jurkat nuclear extract.| Product Name | CtBP Antibody (H-440) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-440 representing full length CtBP1 of human origin |
| Modification Notes | CtBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CtBP1 and the related protein CtBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T-cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of ∂EF1, a cellular zinc finger-homeodomain protein, and thereby enhances ∂EF1 induced transcriptional silencing. In addition, CtBP complexes with CtIP, a protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. CtIP/CtBP binding to BRCA1 inhibits the transactivation of the p21 promoter, and it is critical for regulating p21 transcription in response to DNA damage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CtBP1 and CtBP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-440 representing full length CtBP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-440 representing full length CtBP1 of human origin |
| Entrez Gene | 1487 |
|---|
Application
Western blot analysis of CtBP1 expression in non-transfected: (A) and human CtBP1 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of CtBP1 expression in non-transfected: (A) and mouse CtBP1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.
Application
Western blot analysis of CtBP expression in Jurkat nuclear extract.| Application Notes | recommended for detection of CtBP1 and CtBP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
