Application
Western blot analysis of human recombinant CRBP III fusion protein.Application
Western blot analysis of CRBP III expression in non-transfected: (A) and human CRBP III transfected: (B) 293T whole cell lysates.| Product Name | CRBP III Antibody (FL-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-135 representing full length CRBP III of human origin |
| Modification Notes | The cellular retinol-binding proteins (CRBP I, II, III and IV) belong to a superfamily of small cytoplasmic proteins which interact with hydrophobic ligands. Vitamin A, a molecule essential for cell growth and differentiation, embryonic development and vision, is transported into the cell by the CRBPs in its alcoholic form, called retinol. Both CRBP I and II are composed of ten antiparallel ∫-strands, which form a ∫-barrel that contains the retinol molecule, and two å-helices, which cover the open ends of the barrel. CRBP I mediates the cellular uptake of retinol, solubilizes and detoxifies it for further transport within the cytoplasm, and presents it to the appropriate enzymes to biosynthesize retinoic acid, an active form of retinol or retinyl esters, which are stored. CRBP I is expressed in human ovary, adrenal and pituitary glands, and testis, and its expression is modulated by TGF∫. CRBP II is expressed solely in the small intestine and mediates the absorption of retinoids and carotenoids to biosynthesize retinyl esters. CRBP III and CRBP IV are cytoplasmic proteins that, like CRBP I and CRBP II, form ∫-barrel structures and participate in the intracellular transport of retinol. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CRBP III of human origin by WB, IP, IF and ELISA may cross-react with CRBP I, II and IV also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-135 representing full length CRBP III of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-135 representing full length CRBP III of human origin |
| Entrez Gene | 83758 |
|---|
Application
Western blot analysis of human recombinant CRBP III fusion protein.
Application
Western blot analysis of CRBP III expression in non-transfected: (A) and human CRBP III transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CRBP III of human origin by WB, IP, IF and ELISA may cross-react with CRBP I, II and IV also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
