Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing nuclear and cytoplasmic staining of squamous epithelial cells and glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of p54/nrb expression in non-transfected: (A) and mouse p54/nrb transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of p54/nrb expression in non-transfected 293T: (A), human p54/nrb transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of p54/nrb expression in HeLa (A), MOLT-4 (B) and MCF7 (C) nuclear extracts.| Product Name | p54/nrb Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 381-465 mapping near the C-terminus of p54/nrb of human origin |
| Modification Notes | Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 381-465 mapping near the C-terminus of p54/nrb of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 381-465 mapping near the C-terminus of p54/nrb of human origin |
| Entrez Gene | 4841 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing nuclear and cytoplasmic staining of squamous epithelial cells and glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of p54/nrb expression in non-transfected: (A) and mouse p54/nrb transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of p54/nrb expression in non-transfected 293T: (A), human p54/nrb transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of p54/nrb expression in HeLa (A), MOLT-4 (B) and MCF7 (C) nuclear extracts.| Application Notes | recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
