Application
Western blot analysis of TRB-3 expression in non-transfected: (A) and human TRB-3 transfected: (B) 293T whole cell lysates.| Product Name | TRB-3 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-140 mapping at the N-terminus of TRB-3 of human origin |
| Modification Notes | TRB-3 (tribbles 3), also called NIPK (neuronal cell death-inducible protein kinase) disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRB3 binds to ATF4, inhibiting its transcriptional activation activity, and regulates activation of MAP kinases. In the liver, TRB-3 is a target for PPAR-alpha. Amounts of TRB3 RNA and protein are higher in livers of diabetic mice compared with those in wildtype mice. TRB3 contributes to insulin resistance in individuals with susceptibility to type II diabetes. Highest expression of TRB-3 is in liver, pancreas, peripheral blood leukocytes and bone marrow. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRB-3 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 mapping at the N-terminus of TRB-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 mapping at the N-terminus of TRB-3 of human origin |
| Entrez Gene | 57761 |
|---|
Application
Western blot analysis of TRB-3 expression in non-transfected: (A) and human TRB-3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TRB-3 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
