Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Western blot analysis of ACO2 expression in non-transfected 293T: (A), human ACO2 transfected 293T: (B) and Daudi (C) whole cell lysates.Application
Western blot analysis of ACO2 expression in non-transfected 293T: (A), human ACO2 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of ACO2 expression in mouse heart tissue extract.Application
Western blot analysis of ACO2 expression in non-transfected (A) and human ACO2 transfected (B) 293 whole cell lysates.Application
Western blot analysis of ACO2 expression in non-transfected: (A) and mouse ACO2 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | ACO2 Antibody (A-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ACO2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | ACO2, also referred to as aconitate hydratase, citrate hydrolyase or aconitase, is an iron-sulfur hydrolyase that catalyzes the non-limiting interconversion of citrate and isocitrate in the tricarboxylic acid cycle. It is expressed in the mitochondria and maintains a citrate:isocitrate ratio of approximately 10:1. ACO2 contains a redox-sensitive iron-sulfur cluster that exists in two states: active (Fe4S4) and inactive (Fe3S4). ACO2 activity is dependent on the state of this cluster as well as the presence of two conserved cysteine residues. In normal prostate epithelial cells ACO2 activity is prevented due to the high levels of zinc inhibiting the enzyme. In these citrate-producing epithelial cells citrate oxidation is impaired allowing citrate to accumulate and exhibit a citrate:isocitrate ratio of approximately 30:1. In malignant prostate cells zinc is unable to accumulate, therefore ACO2 activity resumes and citrate is oxidized. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| See 1 citations for ACO2Antibody (A-22): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ACO2 of mouse rat and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ACO2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 50 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of ACO2 expression in non-transfected 293T: (A), human ACO2 transfected 293T: (B) and Daudi (C) whole cell lysates.
Application
Western blot analysis of ACO2 expression in non-transfected 293T: (A), human ACO2 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of ACO2 expression in mouse heart tissue extract.
Application
Western blot analysis of ACO2 expression in non-transfected (A) and human ACO2 transfected (B) 293 whole cell lysates.
Application
Western blot analysis of ACO2 expression in non-transfected: (A) and mouse ACO2 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | See 1 citations for ACO2Antibody (A-22): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
