Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of Dyrk1B expression in non-transfected: (A) and human Dyrk1B transfected: (B) 293T whole cell lysates and Sol8 nuclear extract (C).Application
Western blot analysis of Dyrk1B expression in non-transfected: (A) and human Dyrk1B transfected: (B) 293T whole cell lysates.| Product Name | Dyrk1B Antibody (H-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-45 mapping at the N-terminus of Dyrk1B of human origin |
| Modification Notes | Dyrk (for dual specificity tyrosine phosphorylation regulated kinase) is the homolog of the Drosophila mnb (minibrain) gene which is required for neurogenesis. Dyrk is a dual-specificity tyrosine kinase and serine/threonine kinase, which is self regulated by tyrosine phosphorylation. Several related mammalian proteins compose the Dyrk family of dual specificity protein kinases, including Dyrk1A, Dyrk1B, Dyrk1C, Dyrk2, Dyrk3, Dyrk4A and Dyrk4B. The Dyrk family members are thought to be involved in the regulation of cellular growth and/or development. Dyrk1B localizes to the nucleus in muscle and testis. Specifically, Dyrk1B plays a critical role in muscle differentiation by regulating motility, transcription, cell cycle progression and cell survival. Dyrk1B is also found is several solid tumors, where it acts as a downstream effector of Rac1 or K-ras to mediate cell survival. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dyrk1B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-45 mapping at the N-terminus of Dyrk1B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-45 mapping at the N-terminus of Dyrk1B of human origin |
| Entrez Gene | 9149 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of Dyrk1B expression in non-transfected: (A) and human Dyrk1B transfected: (B) 293T whole cell lysates and Sol8 nuclear extract (C).
Application
Western blot analysis of Dyrk1B expression in non-transfected: (A) and human Dyrk1B transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Dyrk1B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
