Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of MetRS expression in HeLa (A) and SW480 (B) whole cell lysates.| Product Name | MetRS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 121-420 mapping within an internal region of MetRS of human origin |
| Modification Notes | MetRS is a cytoplasmic methionyl-tRNA synthetase. It belongs to the class-I aminoacyl-tRNA synthetase family and contains one GST-like domain and one WHEP-TRS domain. Methionyl-tRNA synthetase is an enzyme that catalyzes the esterification of a Met residue to its compatible cognate tRNA to form Met-tRNA. This enzyme performs its functions with high precision by synchronously recognizing the anticodon region and the aminoacylation region, which are separated by approximately 70 angstroms in space. MetRS is thereby considered allosteric, in that its trinucleotide anticodons bind the enzyme at a site removed from its catalytic domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MetRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-420 mapping within an internal region of MetRS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-420 mapping within an internal region of MetRS of human origin |
| Entrez Gene | 4141 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of MetRS expression in HeLa (A) and SW480 (B) whole cell lysates.| Application Notes | recommended for detection of MetRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
