Application
Western blot analysis of Sulf-1 expression in non-transfected 293T: (A), human Sulf-1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | Sulf-1 Antibody (H-81) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 481-561 mapping within an extracellular domain of Sulf-1 of human origin |
| Modification Notes | Sulf-1 (sulfatase 1), also known as HSULF-1, is an 871 amino acid protein that localizes to both the endoplasmic reticulum and the golgi apparatus and belongs to the sulfatase family. Members of the sulfatase family each contain a conserved active site with a posttranslationally generated alpha-formylglycine that is essential for their catalytic activity. These enzymes are responsible for the hydrolysis of sulfate ester bonds. Sulf-1 and Sulf-2 (sulfatase 2) specifically interact with heparin sulfate proteoglycans (HSPGs) and hydrolyze glucosamine-6-sulfate modifications, thus regulating the interactions of HSPGs with a variety of signaling molecules. As key components of cell surfaces and extracellular matrices, HSPGs modulate growth factor activities and thereby influence cell growth and differentiation. Additionally, HSPGs play a critical role in regulating tumor cell metastasis by mediating cell adhesion and the activities of growth and angiogenic factors. This suggests an important role for Sulf-1 and Sulf-2 in tumor progression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Sulf-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 481-561 mapping within an extracellular domain of Sulf-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 481-561 mapping within an extracellular domain of Sulf-1 of human origin |
| Entrez Gene | 23213 |
|---|
Application
Western blot analysis of Sulf-1 expression in non-transfected 293T: (A), human Sulf-1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Sulf-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
