Application
Western blot analysis of Smurf1 expression in mouse kidney tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | Smurf1 Antibody (X-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Smurf1 of mouse and human origin by WB, IF, IHC(P), ELISA |
| Modification Notes | Smurf1 and Smurf2 (SMAD ubiquitination regulatory factor-1 and 2) are members of the Hect family of proteins, which also includes the ubiquitin (Ub) E3-type ligases Nedd3 and E6-AP (1,2). E3 ligases are involved in the enzymatic reactions of the Ub conjugating pathway, which targets proteins for degradation by the 26S proteasome (3). Within the Ub pathway, the E3 ligases specifically catalyze the transfer of Ub from the Ub-conjugating enzymes to the individual protein substrate (4). As an E3 ligase, Smurf1 selectively interacts with receptor-regulated SMADs specific to the BMP pathway in order to trigger their ubiquitination and degradation (1). Smurf2 interacts with receptor-activated Smads (R-Smads), including Smad1, Smad2, and Smad3, but not Smad4 (5). Although Smurf2 localizes to the nucleus, binding to Smad7 induces its export and its recruitment to the activated TGF∫ receptor, where it causes degradation of Smad7 (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCELISA |
| WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Smurf1 of mouse and human origin by WB IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Smurf1 of mouse and human origin by WB, IF, IHC(P), ELISA |
| Entrez Gene | 57154 |
|---|
Application
Western blot analysis of Smurf1 expression in mouse kidney tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
