Application
Western blot analysis of Neuralized-2 expression in human fetal muscle tissue extract.| Product Name | Neuralized-2 Antibody (J-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Neuralized-2 of human origin by WB, IP and ELISA |
| Modification Notes | Neuralized-2, also known as NEURL2, OZZ or Ozz-E3, is a 285 amino acid cytoplasmic protein involved in myofiber differentiation and maturation that is induced during differentiation. Expressed predominantly in skeletal and cardiac muscle, Neuralized-2 localizes to the tips of differentiating myofibers and regulates ubiquitination and degradation of ∫-catenin at the sarcolemma. Neuralized-2 contains one SOCS box domain, through which it interacts with Elongin B and Elongin C, and one NHR (neuralized homology repeat) domain that is implicated in ubiquitination substrate binding. The gene encoding Neuralized-2 maps to human chromosome 20, which is comprised of approximately 2% of the human genome, contains nearly 63 million bases and encodes over 600 genes. Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis and Alagille syndrome are associated with chromosome 20. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Neuralized-2 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Neuralized-2 of human origin by WB, IP and ELISA |
| Entrez Gene | 140825 |
|---|
Application
Western blot analysis of Neuralized-2 expression in human fetal muscle tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
