Application
Western blot analysis of DDT expression in non-transfected: (A) and human DDT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DDT expression in non-transfected: (A) and human DDT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DDT expression in HeLa whole cell lysate.Application
Western blot analysis of DDT expression in non-transfected 293T: (A), human DDT transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | DDT Antibody (E-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of DDT of human origin |
| Modification Notes | Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. The only known family member of MIF is D-dopachrome tautomerase (DDT), a protein that is thought to similarly play a role in the inflammation process. DDT is highly expressed in liver with lower levels in other organs, including heart, lung and pancreas. It resides in the cytoplasm as a homotrimer and converts 2-carboxy-2,3-dihyroindole-5, 6-quinone (D-dopachrome) into 5,6-dihydroxyindole. DDT requires the presence of an N-terminal proline residue for catalytic activity and is involved in the biosynthesis of melanin, an antioxidant. In response to liver damage, DDT has been shown to increase protein levels in order to accelerate melanin biosynthesis and protect the liver from oxidative stress. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DDT and DDTL of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of DDT of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of DDT of human origin |
| Entrez Gene | 1652 |
|---|
Application
Western blot analysis of DDT expression in non-transfected: (A) and human DDT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DDT expression in non-transfected: (A) and human DDT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DDT expression in HeLa whole cell lysate.
Application
Western blot analysis of DDT expression in non-transfected 293T: (A), human DDT transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of DDT and DDTL of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
