Application
Western blot analysis of melusin expression in human fetal heart tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing nuclear and cytoplasmic localization.| Product Name | melusin Antibody (A-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of melusin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Melusin is a ysteine-rich cytoplasmic protein that is predominantly expressed in striated skeletal and cardiac muscles. Within muscle tissues, Melusin directly associates with the cytoplasmic domains of various subunits of the integrin membrane receptor. Melusin is localized in rows flanking the Z line containing -actinin, which suggests that Melusin, together with the integrin receptors, contributes to the actin-integrin junctional complex and the integrity of the cytoskeleton. Melusin expression is detected in 15 day embryos and it is also highly expressed during secondary myogenesis, a process in which a distinct myoblast population line up using primary myotubes as scaffold and fuse to each other forming secondary myotubes that will give rise to the muscle fibers of adult tissue. In adult tissues, high expression of Melusin is observed in regenerating adult tibialis anterior muscle, further indicating that Melusin contributes to the maturation and organization of muscle cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of melusin of mouse rat and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of melusin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 26548 |
|---|
Application
Western blot analysis of melusin expression in human fetal heart tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
