Application
Western blot analysis of CysRS expression in non-transfected: (A) and human CysRS transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing nuclear and cytoplasmic localization.| Product Name | Cytoplasmic CysRS Antibody (H-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Cytoplasmic CysRS of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of tRNA by their cognate amino acid in the initial step of ribosome-dependent protein biosynthesis. Cytoplasmic CysRS (Cysteinyl-tRNA synthetase, cytoplasmic), also known as CARS, is a 748 amino acid member of the class-I aminoacyl-tRNA synthetase protein family. Cytoplasmic CysRS is a monomeric protein that binds one zinc ion per subunit for use as a cofactor. Cytoplasmic CysRS uses ATP to convert L-cysteine and tRNA(Cys) into ADP, a diphosphate and L-cysteinyl-tRNA(Cys). A chromosomal aberration of the gene that encodes Cytoplasmic CysRS is associated with inflammatory myofibroblastic tumors (IMTs). Cytoplasmic CysRS is expressed as two isoforms produced by alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Cytoplasmic CysRS of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Cytoplasmic CysRS of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 833 |
|---|
Application
Western blot analysis of CysRS expression in non-transfected: (A) and human CysRS transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
