Application
Western blot analysis of PSAT1 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.Application
Western blot analysis of PSAT1 expression in non-transfected: (A) and human PSAT1 transfected: (B) 293 whole cell lysates.| Product Name | PSAT1 Antibody (L-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PSAT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | PSAT1 (phosphoserine aminotransferase 1), also known as phosphoserine aminotransferase, phosphohydroxythreonine aminotransferase, endometrial progesterone-induced protein, PSA, PSAT, EPIP or MGC1460, is a 370 amino acid protein belonging to the class-V pyridoxal-phosphate-dependent aminotransferase family and serC subfamily. PSAT1 catalyzes the second step in the L-serine synthesis pathway, converting 3-phosphohydroxypyruvate into 3-phosphoserine. While highly expressed in pancreas, brain, kidney and liver, PSAT1 is expressed at very low levels in colon, testis, thymus and prostate. There are two known isoforms of PSAT1 which result from alternative splicing events, and the gene encoding PSAT1 maps to human chromsome 9q21.2. PSAT1 deficiency can cause phosphoserine aminotransferase deficiency (PSATD), whose symptoms include psychomotor retardation, intractable seizures, acquired microcephaly and hypertonia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PSAT1 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of PSAT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 29968 |
|---|
Application
Western blot analysis of PSAT1 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.
Application
Western blot analysis of PSAT1 expression in non-transfected: (A) and human PSAT1 transfected: (B) 293 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
